| Solution Information | help | |
| Enzyme: | Nuclear receptor subfamily 1 group I member 2 | |
| inhibitor: | BDBM51902 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this dose response counterscreen is to determine whether compounds identified as active in a previous set of experiments entitled, "Luminescence-based primary cell-based high throughput screening assay to identify activators of the Aryl Hydrocarbon Receptor (AHR)" (AID 2796), that confirmed activity in a set of experiments entitled, "Luminescence-based cell-based high throughput confirmation assay for activators of the Aryl Hydrocarbon Receptor (AHR)" (AID 2845), and that were inactive in a set of experiments entitled, "Counterscreen for activators of the Aryl Hydrocarbon Receptor (AHR): luminescence-based cell-based high throughput screening assay to identify activators of the Pregnane X Receptor (PXR)" (AID 434939) were nonselective due to activation of PXR. This cell-based assay (14) measures the ability of compounds to activate Pregnane X Receptor (PXR) nuclear signaling. The assay employs human hepatoma (DPX-2) cells co-transfected with a plasmid w | |
| If you find an error in this entry please send us an E-mail | ||